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Flow cytometry gating strategy used to study TRIM5α-transduced cells. Gated plots for analysis of infection in untransduced or TRIM5α-transduced cells are shown (left to right): cells gated for live singlets, live singlets gated for lymphocytes (Lymphs), human feeders removed from the lymphocyte population by gating for human CD45-negative cells, and TRIM5α-transduced cells from the GFP+ gate as well as untransduced cells from the GFP− gates.
