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Membrane binding assay. Proteins (MBP-VP4 and control MBP) were incubated with liposome preparations and loaded on the bottom of a sucrose gradient. After ultracentrifugation, gradients were fractionated in four different fractions (F1 to F4, from top to bottom) and analyzed by SDS-PAGE. Liposomes banded at F1. Molecular mass markers (in kDa) are indicated on the left.
