FIG 7.

miR-24-3p reverses the suppressive effect of HO-1 induction on PRRSV infection. MARC-145 cells were transfected with 200 nM concentrations of miR-24-3p mimics, miR-24-3p-Mut mimics, NC mimics, or siHO-1 (as a positive control) for 12 h and then infected with either rHP-PRRSV/SD16/EGFP (MOI of 0.01) for 48 h or PRRSV strain SD16 (MOI of 0.01) for 24 h, followed by treatment with CoPP (20 μM) from 1 hpi onward. The percentage of EGFP-positive cells infected with PRRSV 48 hpi was measured by flow cytometry (A). PRRSV replication was evaluated by qRT-PCR for extracellular PRRSV ORF7 RNA and virus titers in the supernatants (B) and for intracellular PRRSV ORF7 RNA (C). (D) Western blotting for intracellular PRRSV N protein was performed. α-Tubulin was used as the loading control. Results in panels A to C are expressed as means ± standard deviations of three independent experiments. P values were calculated using Student's t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.