FIG 8.

miR-24-3p reverses the suppressive effect of HO-1 induction on PRRSV replication in PAMs. PAMs were transfected with 200 nM concentrations of miR-24-3p mimics, miR-24-3p-Mut mimics, NC mimics, or siHO-1 (as positive control) for 12 h and then infected with PRRSV strain SD16 (MOI of 0.01) for 24 h, followed by treatment with CoPP (5 μM) from 1 hpi onward. PRRSV replication was evaluated by qRT-PCR for extracellular PRRSV ORF7 RNA in the supernatants (A) and for intracellular PRRSV ORF7 RNA (B). Western blotting for intracellular PRRSV N protein was performed, with α-tubulin used as the control (C). Results are expressed as means ± standard deviations of three independent experiments. P values were calculated using Student's t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.