FIG 3.

The expression of hNCL increases EV71 binding to a mouse cell line. NIH 3T3 cells were transfected without vector (No vector), an empty vector (Vector), or the vector containing the hncl gene tagged with FLAG (NCL1 and NCL2). (A) The expression of FLAG on the cell surface was determined by flow cytometry. A histogram (top panel) and the quantitative results of the mean fluorescence intensity (MFI) of FLAG-positive cells are shown. (B) The expression levels of NCL, FLAG, and β-actin in cells were detected by Western blotting. (C) The binding of different genotypes of EV71 (MP4, C2, B4, C4, and B5 strains) to RD cells, NIH 3T3 cells transfected with an empty vector (Vector), and NIH 3T3 (NCL2) cells was determined by flow cytometry. The expression of NCL and β-actin in cells detected by Western blotting (D) and the binding of different genotypes of EV71 (MP4, B4, C4, and B5 strains) to RD cells, L929 cells transfected with an empty vector (Vector), and L929 (NCL) cells determined by flow cytometry (E) are shown. The value of MP4-infected RD cells was defined as 100%. The data are representative of the means ± standard deviations (error bars) of ≥3 samples per group. *, P < 0.05; ***, P < 0.001.