FIG 6.

NCL and SCARB2 associate in uninfected cells, and EV71 infection increases the level of NCL associated with SCARB2 in cells. The lysates of RD cells infected without or with 87-N6356 were harvested at 3 h p.i. and immunoprecipitated (IP) with anti-SCARB2 antibody (A) or anti-NCL antibody (B) prior to immunoblotting with anti-NCL, anti-SCARB2, or anti-EV71 antibody. (C) The colocalization (white) of EV71 antigen (green), NCL (red), and SCARB2 (blue) in RD cells infected with 87-N6356 was examined using a confocal microscope. The data are representative of three independent experiments.