FIG 1.

SV40 recruits C18 to the virus-induced B12/B14 foci which also harbor membrane penetration-competent virus. (A) CV-1 cells were transfected with S-tagged C18, and at 12 h after transfection, cells mock infected or infected with SV40 (MOI, ∼50) for 2 h and 14 h were fixed, stained with anti-S antibody and anti-BAP31 antibodies, and analyzed by immunofluorescence microscopy. (B and C) CV-1 cells were transfected with FLAG-tagged C18. At 12 h after transfection, cells infected with SV40 (MOI, ∼50) for 14 h were fixed, stained with anti-FLAG and anti-B12 (B) or anti-FLAG and anti-B14 antibodies (C), and analyzed by immunofluorescence microscopy. DAPI, 4′,6-diamidino-2-phenylindole (DAPI). (D and E) CV-1 cells were transfected with FLAG-tagged C18 and infected with SV40 (MOI, ∼50) for 14 h, fixed, stained with anti-FLAG and anti-VP1 (D) or anti-FLAG and anti-VP2/3 antibodies (E), and analyzed by immunofluorescence microscopy. (F) Quantification of the percentage of FLAG-C18 foci colocalizing with VP1 or VP2/3 foci. Values represent means ± SD from three independent experiments.