FIG 5.

C18, B12, and B14 play nonredundant roles during SV40 infection. (A, B, and C) CV-1 cells were reverse transfected with scrambled or the indicated siRNAs for 24 h prior to transfection with the indicated FLAG-tagged constructs for 24 h. Cells then were infected with SV40 (MOI, ∼0.5) for 20 h, fixed, and stained with anti-FLAG and anti-large T antigen antibodies. The percentages of T antigen-positive cells were determined in GFP-, C18-, B12-, or B14-expressing cells by using immunofluorescence microscopy. Values represent means ± SD from three independent experiments. (D) CV-1 cells were transfected with scrambled or the indicated siRNAs for 48 h prior to infection with SV40 (MOI, ∼0.5) for 20 h. Cells were fixed and stained with anti-large T antigen antibodies, and the percentages of large T antigen-positive cells were scored by using immunofluorescence microscopy. Values represent means ± SD from three independent experiments.