Figure 4.
Effect of H2S on Cd-induced ROS accumulation in Chinese cabbage roots. (a) Histochemical detection of H2O2. (b) Histochemical detection of O2 •−. (c) Fluorescence probe staining of ROS observed under fluorescence microscopy at 488 nm and relative fluorescence intensity of ROS. CK: control; NaHS: fumigated with 5 μM NaHS for 24 h; Cd: 5 mM Cd stressed for 48 h; NaHS + Cd: seedlings fumigated with 5 μM NaHS for 24 h and then treated with 5 mM Cd for 48 h; HA + Cd: seedlings treated with 1 mM HA for 4 h and then treated with 5 mM Cd for 48 h; HA: seedlings treated with 1 mM HA for 4 h. Data are mean ± SE of three independent repeats. LSD was used for multiple comparisons; different letters indicate significant differences (P < 0.05).