FIG 1.

Production and characterization of JEV g3 and g5 viral stocks. JEV g3 and g5 were produced after transfection of infectious DNA into mammalian cells, followed by amplification of viral stocks in C6/36 cells. (A) Example of infectious foci developed for JEV g3 and g5 after FFA in BHK-21 cells. (B) Qualification of viral stocks grown from C6/36 cells: infectious virus contents of the supernatants were analyzed by titration on BHK-21 cells (dashed bars), while viral RNA was quantified by RT-qPCR (gray bars). A representative experiment (n > 3) is shown. The error bars represent the standard deviations of the means (the assays were done in duplicate). (C) The specific infectivity was calculated as the number of viral RNA molecules per infectious unit of each virus.