FIG 2.

BDV infection decreases histone acetylation levels in persistently infected Vero cells. (A) Analysis of H2B acetylation. (Left panel) Histone fractions were prepared from parallel noninfected (NI) or persistently infected (BDV) Vero cells and analyzed by Western blotting with the indicated antibodies. Total H2B levels were assessed for each sample and used to normalize acetylation signals. (Right panel) Quantification of H2B acetylation. Acetylation values (normalized for total H2B) obtained for infected cells were normalized to the values obtained for noninfected cells, which were set to 1 and which are represented by the dashed lines on the graphs. Data are expressed as means ± SEM of the results from at least seven independent experiments, i.e., using cultures of Vero cells that were independently grown after the original split during 5 to 20 passages. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ns = nonsignificant (by paired t test). (B) Analysis of H3 and H4 global acetylation by Western blotting, done as described for panel A. (C) Western blot analysis of acetylation on each H4 lysine residues, done as described for panel A.