FIG 7.

The immunopeptidome reflects changes of the cellular proteome in MVA.HIVconsv-infected cells. (A) The most relevant pathways that are affected by MVA.HIVconsv infection at 3.5 hpi, as identified by Ingenuity Pathway Analysis, for all proteins according to the abundances of the corresponding HLA-associated peptides (left) and the intracellular proteins (right), as determined by label-free quantitative LC-MS/MS. All changes in abundance were determined between the 3.5-h time point and the negative control. (B) Correlation factors between the trend of protein abundance and the trend of the HLA-associated peptide abundance at 0, 1.5, 2.5, and 3.5 hpi. A proportional correlation was assigned a value of +1, and an inverse correlation was assigned a value of −1. Factors are plotted for all proteins along the x axis, for which both protein and immunopeptide abundances were determined.