FIG 1.

Chaotrope effects on the binding of gp120-specific MAbs and lectins. The MAbs and lectins were titrated against D7324-captured gp120 (JR-FL) in ELISAs. Quantification of bound MAb, detected as luminescence (relative light units [RLU]), is expressed on the y axis as a function of MAb concentration (ng/ml) on the x axis. Ligand binding after chaotrope (NaSCN) or control (PBS) treatment is represented by dashed and solid lines, respectively. The data points are the mean results from three intra-assay replicates, and the error bars show the standard errors of the means (SEM). Each plot is representative of ≥2 replicate experiments.