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. 2015 Mar 25;89(11):5919–5934. doi: 10.1128/JVI.00463-15

FIG 5.

FIG 5

Transfection of in vitro-transcribed CrPV-2 and CrPV-3 RNAs in S2 cells. (A) Phase-contrast images of S2 cells mock transfected, CrPV infected (MOI of 0.4), or transfected with in vitro-transcribed CrPV-2, CrPV-3, CrPV-2-ORF1-STOP, or CrPV-2-ORF2-STOP RNA at 48 h posttransfection (hpt). (B) Northern blots of CrPV RNA genome from RNA isolated from CrPV-infected S2 cells or cells transfected with the indicated CrPV genomic RNA. Methylene blue staining of blots are shown below. (C) RT-PCR analysis of CrPV negative-strand (−) synthesis from RNA of cells transfected with the indicated CrPV genomic RNA or CrPV-infected at 24 hpt. Results of reactions in the presence (+RT) or absence (−RT) of reverse transcriptase are shown. (D) Western blots of viral 3CD (ORF1) and VP2 (ORF2) from lysates of cells transfected with the indicated CrPV genomic RNA or CrPV infected at 48 hpt.