FIG 8.

CrPV infectious clones can infect Drosophila S2 cells devoid of Drosophila C virus, flock house virus, and Drosophila X virus. (A) Detection of Drosophila C virus (DCV), flock house virus (FHV), and Drosophila X virus (DXV) by RT-PCR analysis in UBC S2 cells, Kc167 cells, Invitrogen S2 cells, and S2 cells from the Drosophila Genomics Resource Center. (B) Northern blots of CrPV RNA genome from RNA isolated from Kc167 cells and S2 cells from Invitrogen that were transfected with the indicated CrPV genomic RNA. The presence (+) or absence (−) of DCV, FHV, and DXV is indicated for each cell line. Methylene blue staining of blots is shown below.