FIG 3.
Relative infectivity of HIV-1NL4-3 and its mutant viruses. The wild-type HIV-1NL4-3 and mutant pseudoviruses were normalized to a fixed amount by p24 antigen, and viral infectivity was tested in TZM-bl cells using a single-cycle infection assay. The luciferase activity was measured and corrected for background. The luciferase activity of HIV-1NL4-3 was treated as 100%, and the relative infectivity of other mutant viruses was calculated accordingly. Data were derived from the results of three independent experiments and are expressed as means ± standard deviations.