FIG 6.

Effects of the E49K and N126K substitutions on the conformation of 6-HB structure. The reactivity of the 6-HBs formed by N36 and C34 or their mutants with conformation-dependent MAbs NC-1 (A) and 17C8 (B) was tested by ELISA. The peptide mixture was coated on the plate wells at 10 μg/ml, and the final concentration of a tested MAb was 5 μg/ml. Data were derived from three independent experiments and are expressed as means ± standard deviations. OD₄₅₀, optical density at 450 nm.