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Binding stability of truncated short-peptide inhibitors determined by CD spectroscopy. The thermostability of 6-HBs formed by MTSC21 (A), MTSC19 (B), HP22 (C), or HP23E (D) with N36 and N36E49K was measured. The Tm value was defined as the midpoint of the thermal unfolding transition. The final concentration of each peptide in PBS is 10 μM. The experiments were repeated at least three times, and representative data are shown.
