Skip to main content
. 2015 Mar 18;89(11):5835–5846. doi: 10.1128/JVI.03137-14

TABLE 1.

Details of PB1-F2 mutants made in this studya

Source virus Name of mutant PB1-F2 status or size (aa) Mutagenesis primer(s)
VN/281 VN/281/PB1-F2FL Full length (90) Kpn1PB1F76-94 and Xba1PB1R384-67
VN/281/PB1-F2TR Truncatedb (24) PB1C167T_C170T_F
VN/281/PB1-F2NO Deletedc PB1T96C _F
VN/281/PB1-F2FL-HA Full length (90) Kpn1PB1F68-90 and Xba1HAtagPB1R364-43
VN/281/PB1-F2HA-TR Truncatedb (24) PB1F151-185 (HA_C167T_C170T)
VN/281/PB1-F2TR-HA Truncatedd PB1C167T_C170T_F and Xba1HAtagPB1R364-43
VN/281/PB1-F2NO-HA Deletedc PB1T96C _F
VN/296 VN/296/PB1-F2FL Full length (90) PB1C96T_F
VN/296/PB1-F2TR Truncated (24) PB1C167T_C170T_F
VN/296/PB1-F2NO Deletedc Kpn1PB1F76-94 and Xba1PB1R384-67
VN/296/PB1-F2FL-HA Full length (90) PB1C96T_F
VN/296/PB1-F2HA-TR Truncatedb (24) PB1F151-185 (HA_C167T_C170T)
VN/296/PB1-F2TR-HA Truncatedd PB1C167T_C170T_F and Xba1HAtagPB1R364-43
VN/296/PB1-F2NO-HA Deletedc Kpn1PB1F68-90 and Xba1HAtagPB1R364-43
a

A total of 6 mutants were made from each virus: 3 with a C-terminal HA tag and 3 without the HA tag. See Fig. 1 for schematic diagrams.

b

Stop codons were added at positions 25 and 26; the plasmid is expected to express a truncated protein (24 aa). One-half of these mutants have the HA tag immediately upstream from the stop codons.

c

In this variant, only the first ATG is mutated to ACG, leaving the downstream in-frame ATGs intact.

d

This variant has stops at positions 25 and 26, but the HA tag is at the C terminus.