TABLE 3.
Amino acid differences between viruses VN/281 and VN/296a
| Protein | Size (aa) | No. of variations | Variant residues (VN/281→VN/296) |
|---|---|---|---|
| HA | 567 | 1 | Q31R |
| M1 | 252 | 0 | |
| M2 | 97 | 2 | G21V, L86V |
| NA | 449 | 4 | I63V, G85S, I203T, T244I |
| NP | 498 | 1 | A373T |
| NS1 | 225b | 1 | D120G |
| NS2 | 121 | 0 | |
| PA | 716 | 2 | S66G, A323V |
| PB1 | 756 | 3 | I181M, P369S, V667I |
| PB1-F2 | 90c | 3 | M1T, Q54R, P67L |
| PB2 | 759 | 2 | D304E, A588V |
| Total | 4,530 | 19 | |
| Total (excluding PB1-F2) | 4,440 | 16 |
a
Amino acid differences for each protein were calculated from the nucleotide sequences of the respective ORFs using the Cubit application in BioEdit software.
b
Both viruses have a C-terminal truncation in NS1 that renders it 215 aa long.
c
PB1-F2 is 90 aa in virus VN/281, while the initiating ATG is mutated to ACG in virus VN/296. The 3 differences mentioned in the table are counted since a protein may express either from alternating start codons or from downstream in-frame ATGs.