FIG 2.

RNP localization in different IV-infected cell lines treated with or without caspase 3/7 inhibitor. (A) A range of permanent cell lines were infected with FPV (MOI = 1) and treated with or without caspase 3/7 inhibitor (CI) immediately after infection. Intracellular RNP localization was detected at 8 h p.i. by confocal laser scanning microscopy using anti-NP specific Ab (green). DNA was stained with DAPI (blue). (B) Primary human alveolar epithelial cells (hAEC) were infected with H1N1pdm09 (MOI = 0.5) and treated with or without CI immediately after infection. Intracellular RNP localization was detected at 6, 8, and 10 h p.i. by confocal laser scanning microscopy using anti-NP specific Ab (green). DNA was stained with DAPI (blue).