TABLE 1.
Association of CD8+ T cell responses to 19 epitopes with pVL and CD4 count in chronically HIV-1-infected Japanese individuals
| Epitope | Sequence | Location | HLA restriction | Frequency |
Median pVL (copies/ml) |
Median CD4 (cells/μl) |
P valuea |
q valueb |
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Responders | Nonresponders | Responders | Nonresponders | Responders | Nonresponders | pVL | CD4 | pVL | CD4 | ||||
| NefTY11 | TQGYFPDWQNY | Nef117-127 | B*15:01 | 3 | 390 | 3,400 | 25,000 | 386 | 321 | 0.22 | 0.38 | 0.25 | 0.4 |
| GagEM11 | EGATPQDLNTM | Gag177-187 | B*67:01 | 7 | 386 | 2,200 | 25,000 | 440 | 321 | 0.011 | 0.025 | 0.024 | 0.044 |
| C*08:01 | 2 | 391 | 29,000 | 25,000 | 451 | 322 | 0.86 | 0.36 | 0.86 | 0.4 | |||
| GagTL9 | TPQDLNTML | Gag180-188 | B*67:01 | 15 | 378 | 8,900 | 25,000 | 440 | 319 | 0.11 | 6.5 × 10−3 | 0.14 | 0.015 |
| GagMI8 | MQMLKETI | Gag198-205 | B*52:01 | 42 | 351 | 5,350 | 27,000 | 437 | 313 | 3.3 × 10−4 | 4.9 × 10−4 | 1.6 × 10−3 | 2.3 × 10−3 |
| GagQA11 | QMLKETINEEA | Gag199-209 | B*52:01 | 11 | 382 | 3,100 | 26,500 | 432 | 321 | 4.2 × 10−5 | 0.044 | 2.7 × 10−4 | 0.07 |
| GagRI8 | RMYSPTSI | Gag275-282 | B*52:01 | 60 | 333 | 10,650 | 27,000 | 446 | 309 | 2.1 × 10−3 | 3.2 × 10−7 | 6.6 × 10−3 | 6.1 × 10−6 |
| GagYL9 | YVDRFYKTL | Gag296-304 | C*03:04 | 2 | 391 | 2,885 | 25,000 | 303 | 322 | 0.079 | 0.79 | 0.12 | 0.79 |
| GagWV8 | WMTETLLV | Gag316-323 | B*52:01 | 43 | 350 | 4,700 | 28,000 | 446 | 309 | 1.0 × 10−6 | 8.7 × 10−7 | 1.9 × 10−5 | 8.3 × 10−6 |
| GagNL11 | NPDCKTILKAL | Gag327-337 | B*67:01 | 9 | 384 | 2,200 | 25,500 | 440 | 321 | 2.4 × 10−3 | 0.02 | 6.6 × 10−3 | 0.038 |
| GagGM9 | GPAATLEEM | Gag338-346 | B*67:01 | 1 | 392 | 780 | 25,000 | 693 | 322 | 0.14 | 0.11 | 0.16 | 0.15 |
| GagAA9 | ATLEEMMTA | Gag341-349 | A*02:06 | 30 | 363 | 8,650 | 27,000 | 437 | 314 | 0.019 | 6.6 × 10−4 | 0.036 | 2.5 × 10−3 |
| GagKL9 | KELYPLASL | Gag481-489 | B*40:02 | 12 | 381 | 20,000 | 25,000 | 480 | 321 | 0.68 | 0.017 | 0.72 | 0.035 |
| PolSV9 | SQIYAGIKV | Pol423-431 | A*02:06 | 48 | 345 | 15,000 | 27,000 | 392 | 313 | 0.04 | 4.6 × 10−3 | 0.064 | 0.015 |
| PolSI8 | SQYALGII | Pol654-661 | B*52:01 | 43 | 350 | 5,000 | 28,000 | 456 | 310 | 1.3 × 10−5 | 2.5 × 10−6 | 1.3 × 10−4 | 1.6 × 10−5 |
| PolLA9 | LEGKIILVA | Pol783-791 | B*40:06 | 16 | 377 | 4,150 | 27,000 | 437 | 321 | 5.6 × 10−4 | 0.078 | 2.1 × 10−3 | 0.11 |
| PolIT10 | IEAEVIPAET | Pol799-808 | B*40:06 | 25 | 368 | 13,000 | 26,500 | 353 | 321 | 4.5 × 10−3 | 0.19 | 0.011 | 0.22 |
| PolGI8 | GERIVDII | Pol912-919 | B*40:02 | 39 | 354 | 14,000 | 28,000 | 405 | 317 | 0.021 | 5.5 × 10−3 | 0.036 | 0.015 |
| PolTL8 | TDIQTKEL | Pol921-928 | B*40:02 | 5 | 388 | 7,200 | 25,000 | 478 | 322 | 0.11 | 0.17 | 0.14 | 0.22 |
a
The statistical analyses of differences in pVL or CD4 count between responders to each epitope and nonresponders were conducted by using the two-tailed Mann-Whitney test. Bold indicates that differences were statistically significant.
b
Multiple tests were performed by using the q value, a measure of significance in terms of the false-discovery rate (46). A significance threshold for q of <0.2 was employed.