FIG 3.

vBcl-2 is essential for KSHV lytic gene expression in iSLK cells. (A) iSLK-BAC16-vBcl-2-HA cells were induced by doxycycline (Dox) and sodium butyrate, and whole-cell lysates (WCLs) were collected at 0, 12, 24, and 48 h postinduction and subjected to immunoblotting with the indicated antibodies. (B to D) iSLK-BAC16 (wild type) and iSLK-BAC16-vBcl-2-KO cells were induced by doxycycline and sodium butyrate, and cells were collected at 0, 12, 24, 48, and 72 h postinduction. The whole-cell lysates were subjected to immunoblotting with the indicated antibodies (B); total RNA or DNA was extracted and subjected to qRT-PCR with the indicated primers to examine viral gene expression (C) or viral genome copy number (D), respectively.