FIG 2.

Comparison of the levels of KSHV gene expression in iSLK cells infected with BAC16 and BAC16-mCherry-vBcl-2. iSLK cells harboring BAC16 and BAC16-mCherry-vBcl-2 were induced to undergo lytic virus reactivation using sodium butyrate and doxycycline. At 0, 48, 72, and 96 h following induction, whole-cell lysates were prepared and protein expression by viral genes was analyzed by immunoblotting with the indicated antibodies against viral proteins, as well as GFP. The same blot was reprobed with anti-tubulin as a loading control. Results from one experiment are shown and are representative of those from two experiments providing similar results.