FIG 6.

Comparison of the levels of KSHV DNA replication in iSLK cells infected with wild-type or vBcl-2 stop mutants. High-molecular-weight DNA was extracted at 0, 24, 48, and 72 h postinduction, and the number of KSHV genomes copies relative to the amount of cellular DNA was determined by quantitative TaqMan PCR. Values were normalized to the value for noninduced BAC16 (A) or BAC16-mCherry-vBcl-2 (B), which was defined as 1. The KSHV DNA level was normalized to the level of the human RNase P gene. Results from one experiment are shown and are representative of those from three experiments providing similar results.