FIG 1.

Reductions in EXT1 mRNA and protein levels correlate with reduced infection of MARV and EBOV pseudotyped particles. (a) Introduction of siRNAs of EXT1 resulted in reduced infection by MARVpv and EBOVpv. The siRNAs of EXT1 (labeled as siRNA #1 and #2) were transfected to A549 cells, and their effects on pseudotyped Marburg virus (MARVpv), Ebola virus (EBOVpv), and influenza virus (AIVpv) were evaluated as described in Materials and Methods. A siRNA of firefly luciferase was used as a control in the experiment, and the data were normalized to nontargeting siRNA (NT). Error bars represent standard deviations. (b) Real-time PCR on cells transfected with EXT1 siRNAs showed mRNA knockdown of EXT1 at 48 and 96 h posttransfection, using NT siRNA as a control. Error bars represent standard deviations. (c) The siRNAs of EXT1 reduced the protein level of EXT1 in the target cells. An EXT1-C-Myc plasmid and EXT1 siRNAs were cotransfected to A549 cells, and the EXT1-C-Myc level in the cells was evaluated by Western analysis. In this experiment, different amounts of EXT1-C-Myc plasmid DNA (0.2 to 0.8 μg) were used. β-Actin was used as a control.