FIG 7.

The prM/E heterodimeric interaction and endo-H digestion. (A) The transfected BHK-21 cell lysates as described in the VLP experiments were immunoprecipitated (IP) with mouse E DIII antiserum at 48 h posttransfection and then analyzed by Western blotting for the presence of the E and prM proteins. Normal mouse IgG (Nor-IgG) was used for immunoprecipitation as a negative control; rabbit E DIII antiserum was used for Western blotting to avoid the interference of antibody heavy chain. (B) The cell lysates at 48 h posttransfection were digested with endo-H or left undigested and analyzed by Western blotting using mouse E DIII antiserum. The long exposures for the I₀, ij, BC, and DE mutants are shown below. Results of representative experiment out of at least two independent experiments are shown.