FIG 1.

SEVI fibrils bind HIV-1 and enhance infection in vitro. (A) SEVI fibrils (red) were incubated with HIV-1 (green). (B and C) TZM-bl reporter cells, visualized by Hoechst staining (blue), were incubated with either HIV-1 alone (B) or HIV-1/SEVI complexes obtained from the assay depicted in panel A (C). (D) Effect of SEVI on HIV-1 infectivity. Data were normalized by control conditions (cells only, AZT only, and SEVI only). The dotted line represents the 0-μg/ml SEVI conditions. Fold enhancement of infectivity was obtained from 3 separate experiments performed in triplicate. Arrows in panels B and C highlight HIV-1 on cells. Images were taken at a ×100 magnification. Bars = 5 μm.