TABLE 2.
Effects of seminal plasma treatment on the interaction of HIV with ectocervical tissues from 4 human donors
| Parameter | Value for groupe |
P value | ||||
|---|---|---|---|---|---|---|
| Untreated | Treated with dilution of medium to SP of: |
|||||
| 1:1 | 2:1 | 3:1 | 4:1 | |||
| Median total no. of virions (range)a | 25 (8–34) | 14 (5–18) | 19 (5–23) | 15 (2–59) | 25.5 (18–54) | 0.26 |
| Total no. of virions/total no. of images (no. of virions per image) | 110/93 (1.18) | 41/67 (0.61) | 73/88 (0.83) | 132/98 (1.35) | 137/90 (1.52) | |
| No. (%) of penetrators | 22 (20) | 13 (32) | 11 (15) | 55 (42) | 24 (18) | |
| Prevalence ratio (P value)b | NA | 1.52 (0.13) | 0.72 (0.05) | 1.95 (0.05) | 0.83 (0.54) | |
| Avg penetration depth (μm)c | ||||||
| Including nonpenetrators | 1.28 | 2.86 | 1.41 | 2.55 | 1.19 | 0.08 |
| Among penetrators | 6.39 | 9.03 | 9.35 | 6.11 | 6.80 | 0.65 |
| No. of penetrators <15 μm/total no. of penetrators (%)d | 20/22 (91) | 10/13 (77) | 9/11 (82) | 49/55 (89) | 21/24 (88) | 0.71 |
a
Values shown are medians (ranges [minimum to maximum]); the P value was determined by a Kruskal-Wallis test comparing the distributions of the total number of virions per 20 images in each of 4 donors.
b
The prevalence ratio and P value were estimated from log-binomial regression models weighted by the inverse of the number of images per donor and by using generalized estimating equations to adjust for repeated measures.
c
Average penetration depth per virion over all 4 donors. One-way analysis of variance was performed for comparisons across dilution groups.
d
P value determined by a Fisher exact test.
e
NA, not applicable.