TABLE 3.
Effects of SEVI treatment on the interaction of HIV with ectocervical, endocervical, and vaginal tissues from 8 rhesus macaques
| Parameter | Value for group |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Ectocervix |
Endocervix |
Vagina |
|||||||
| SEVI− | SEVI+ | P | SEVI− | SEVI+ | P | SEVI− | SEVI+ | P | |
| Median total no. of virions (min, max)a | 41.7 (25.4, 246.6) | 28.3 (0, 141.25) | 0.60 | 2.39 (0, 56.25) | 42.1 (4, 135) | 0.11 | 66.6 (34.4, 491.3) | 33.3 (1.5, 215.5) | 0.34 |
| Total no. of virions/total no. of images (no. of virions/image) | 379/455 (0.83) | 181/380 (0.48) | 52/477 (0.11) | 284/500 (0.57) | 1,190/1,059 (1.12) | 602/900 (0.67) | |||
| Median no. of penetrators (min, max)a | 25.3 (11.6, 107.8) | 12.1 (0, 56.3) | 0.43 | 1.0 (0, 30) | 9.3 (2, 135) | 0.20 | 27.1 (14.9, 100.6) | 17.4 (1.5, 58.5) | 0.49 |
| No. of penetrators/total no. of images (no. of penetrators/image) | 179/455 (0.39) | 74/380 (0.20) | 27/477 (0.06) | 122/500 (0.24) | 330/1,059 (0.31) | 207/900 (0.23) | |||
| No. of penetrators/total no. of virions (%) | 179/379 (47) | 74/181 (41) | 27/52 (52) | 122/284 (43) | 330/1,190 (28) | 207/602 (34) | |||
| Prevalence ratio (SEVI+ vs SEVI−) (95% confidence interval)b | 0.85 (0.68, 1.07) | 0.17 | 0.85 (0.48, 1.50) | 0.57 | 1.30 (0.80, 2.10) | 0.29 | |||
| Avg penetration depth (μm)c | |||||||||
| Including nonpenetrators | 3.61 | 2.53 | 0.03 | 10.38 | 9.22 | 0.58 | 2.88 | 2.97 | 0.79 |
| Among penetrators | 7.65 | 6.20 | 0.14 | 19.98 | 21.46 | 0.62 | 10.39 | 8.64 | 0.03 |
| No. of penetrators <15 μm/total no. of penetrators (%)d | 159/179 (89) | 68/74 (92) | 0.47 | 12/27 (44) | 46/122 (38) | 0.52 | 250/330 (76) | 176/207 (85) | 0.01 |
| Max penetration depth (μm) | 36.9 | 28.2 | 41.4 | 57.6 | 47.3 | 45.8 | |||
a
P values were determined by an exact Wilcoxon two-sample test comparing the distribution of the total number of virions or number of penetrators per 100 images for each animal.
b
Prevalence ratios (95% confidence intervals) and P values were estimated from log-binomial regression models weighted by the inverse of the number of images per animal and by using generalized estimating equations to adjust for repeated measures
c
Average penetration depth per virion over all 8 animals. t tests were performed to compare conditions without SEVI to those with SEVI.
d
P values determined by chi-square tests.