FIG 6.
Infection with rILL346 and rILL18 affects pulmonary innate cell trafficking. Pulmonary cellular infiltration in mice infected with rILL346, rILL18, huH1N1, and swH1N2 at days 2 and 4 p.i. BALB/c mice (n = 5) were intranasally infected with 5 × 105 PFU of virus, and BAL fluid was collected on days 2 and 4 p.i. Pan-leukocyte (CD45+) (A), neutrophil (Gr-11hi Ly6Ghi) (B), and NK cell (CD3− DX5+ NK46p+) (C) infiltration was determined by flow cytometry. All data are representative of three individual experiments. Asterisks denote significance related to the pH1N1 parental virus while number signs (#) represent P values significant compared to the swH1N2 parental virus. Results were considered significant where P values were ≤0.05 (*/#), ≤0.01 (**/##), and ≤0.001 (***/###).