FIG 3.
Effect of H2S donor treatment on RSV-induced cytokine and chemokine production in A549 cells. Cells were infected with RSV for 1 h and then incubated in the presence or absence of GYY4137 at 1, 5, and 10 mM for 24 h. Cell supernatants were assayed for cytokine and chemokine secretion by an ELISA or a Bio-Plex assay (A) and for cytotoxicity by an LDH release assay (B). Results are expressed as means ± standard errors and are representative of data from at least three independent experiments run in triplicate for the experiments described above for panel A. *, P < 0.05 compared to untreated RSV-infected cells.