FIG 1.
Survival, body weight, viral burden, and BBB permeability analysis in mice after JEV-P3 infection. (A) Eight-week-old age-matched C57BL/6 mice were intravenously injected with 103 or 105 PFU of JEV-P3. Kaplan-Meier curves show the survival distribution for mice over the 21-day period following challenge (n = 20). (B) Average percent body weight changes were monitored. Body weights were determined every other day, beginning on day 0 until day 14. Mock-infected group, n = 18; infection group, n = 180. (C) The C gene copies of JEV in the brains, spleens, lymph nodes, and livers of JEV-infected mice were measured by qRT-PCR. (D) Evans blue dye was injected intravenously into mice. Representative brains are shown from mock-infected mice or from mice showing mild and severe symptoms. The data are expressed as means ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.005.