FIG 3.

Pathological features of JEV infection in mouse brain. (A) Brain histopathological changes of JEV-infected mice with disease signs were examined by H&E staining at 5 dpi. c, perivascular cuff (black arrows); d, microglia nodule (red arrows). (B) Immunofluorescent triple staining of neuron infection (a to d), astrocyte activation (e to i), and microglial activation (m to t). Brain sections were collected from mock-infected and JEV-infected mice at 5 dpi. E, JEV envelope protein (red). MAP-2, GFAP, and IBA-1 indicate markers for neurons, astrocytes, and microglia, respectively (green). DAPI stains for nuclei (blue). Subpanels e to h and m to p show brain sections from mock-infected mice.