FIG 8.
Effects of anti-IFN-γ neutralizing antibody on the BBB permeability of JEV-infected mice. (A) Eight-week-old age-matched C57BL/6 mice were intravenously injected with 105 PFU of JEV-P3. At 0, 1, 2, and 3 dpi, the mice were intraperitoneally injected with anti-IFN-γ neutralizing antibody or isotype control. At 5 dpi, the BBB permeability was determined with NaF uptake. (B) At 5 dpi, brain tissues were collected to measure the expression of TJ proteins by Western blotting. (C) The results of panel B were normalized with β-actin and densimetrically quantified as the fold change versus a mock-infected control. (D) At 5 dpi, viral loads in the brain were determined with a plaque assay after antibody administration. The data are means ± SEM of the results from three independent experiments. The statistical significance values in panel C were assessed by using two-tailed Student t test. Asterisks indicate the statistical significance (*, P < 0.05; **, P < 0.01).