FIG 2.

The nonpermissive role of KIR3DS1-like residues is dependent on both HLA and peptide. (A to D) HEK293T cells were transfected with FLAG-tagged KIR3DL1*001 or single amino acid variants thereof and stained with individual pHLA class I tetramers as indicated. Results are expressed relative to HLA-A*24:02-RW8 (A) or HLA-B*57:01-TW10 (C) binding to KIR3DL1*001 or relative to the binding of each tetramer to KIR3DL1*001 (B and D). Data are averaged from three independent experiments. Error bars represent standard errors of the means. Binding of each tetramer was compared to that seen with KIR3DL1*001 using a one-way ANOVA, followed by a Dunnett's multiple comparison posttest. *, P < 0.05; **, P < 0.01; ***, P < 0.001.