FIG 1.
Primary cultures of mouse prostate epithelial cells express cytokeratins. Primary cultures of mouse prostate epithelial cells were cultured from normal PtenL/L prostates, Pten−/− prostate tumors, and castration-resistant Pten−/− prostate tumors. Cells were seeded into imaging plates and labeled with DAPI (blue) as a marker for cellular DNA and immunolabeled for cytokeratin (red) as a marker for epithelial cells. Using the DAPI label to define the region of interest to denote single cells, the percentages of cells immunolabeled with cytokeratin were determined and are shown in the graph. Murine embryonic fibroblasts (MEFs) were used as a negative control for cytokeratin immunolabeling.