FIG 3.
Castration-resistant tumor cells are primarily susceptible to VSV infection. Pten−/− mice were castrated at 3 months of age and by 6 months of age had developed castration-resistant tumors. Primary cultures of prostate epithelial cells were established from Pten−/− prostate tumors of 6-month-old mice, castration-resistant tumors, and their littermate normal PtenL/L control prostates. Cells were seeded into imaging plates and infected with rwt-GFP and rM51R-GFP viruses (green). Cells were fixed, permeabilized, immunolabeled for cytokeratin (red) and stained with DAPI to label cellular DNA (blue), and then analyzed with a high-content imaging system. Representative fluorescence images are shown in panels A and B. The numbers of cells that were positive for GFP and cytokeratin were expressed as a percentage of total cytokeratin-positive cells (C and D). The data shown are averages of at least three experiments ± the SEM.