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Tumor-derived Pten−/− cells support very little viral gene expression even at a high MOI. Vector control PtenL/L cells and acutely deleted Pten−/− cells (A) or nontransduced control PtenL/L cells and tumor-derived Pten−/− cells (B) were infected with rwt-dsRed virus at MOIs of 1, 10, and 50. Bright-field and fluorescence images were obtained at 24 h postinfection. The images shown are representative from two to three independent experiments.
