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. 2015 Mar 4;89(10):5462–5477. doi: 10.1128/JVI.03197-14

FIG 5.

FIG 5

Effect of G3BP1 or DDX3X silencing on SG formation and DDX3X–IKK-α localization. Huh-7.5.1 cells were treated with nontargeting control (siNT) or G3BP1 or DDX3X siRNA for 3 days and then infected with JFH-1 HCVcc for 2 days. LDs and various indicated proteins then were stained and analyzed for their subcellular localizations and associations. Magnifications of the selected area are shown on the right. In the majority of siG3BP1- or siDDX3X-treated cells, G3BP1 or DDX3X was efficiently depleted. A few cells still showed G3BP1 or DDX3X expression; therefore, SG-DDX3X-LD colocalization still exists in these cells (A, lower, and B, upper). (A) Small DDX3X granules are associated with LDs in G3BP1-silenced cells. (B) DDX3X depletion abrogated SG formation. (C) Lack of IKK-α granules in G3BP1-silenced cells. Colors of pictures on the far right were modified for better visualization of DDX3X (A), G3BP1 (B), or IKK-α (C), shown in green instead of magenta. Scale bars, 10 μm.