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. 2015 Mar 4;89(10):5491–5501. doi: 10.1128/JVI.00107-15

TABLE 2.

Synthetic oligonucleotide primers used for cloning and expression of mutated and truncated ORF2 proteins of the CaHEV strain and the N protein of the PRRSV SD16 strain

Oligonucleotide Sequenced Motif
aORF2 355-570-Sa CGGGATCCGTGAAATTGTATATGT 355VKLYM
aORF2 360-570-S CGGATCCTCGGTGGAGGATGCTGTC 360SVEDAV
aORF2 365-570-S CGGATCCGTCAACGACAAGCCCATT 365VNDKPI
aORF2 371-570-S CAAGGATCCATGGTCCCTCACGACATTGA 371MVPHD
aORF2 380-570-S GAAGGATCCACTAGCACTGTTACCTGC 380TSTVTC
aORF2 PH373-374RR-Sb AAAGGATCCATGGTCCGTCGCGACATTGAC 371MVRRD
aORF2 P373R-S AAAGGATCCATGGTCCGTCACGACATTGAC 371MVRHD
aORF2 H374R-S CAAGGATCCATGGTCCCTCGCGACATTGAC 371MVPRD
aORF2 V372A-S CAAGGATCCATGGCCCCTCACGACATT 371MAPHD
aORF2 D375E-S CAAGGATCCATGGTCCCTCACGAGATTGACCT 371MVPHE
aORF2-Rc GAACTCGAGGGTGGTGCAATCATCAC
N Protein-S CGGGATCCATGCCAAATAACAACGGCAAG
N Protein-R AACTCGAGTCATGCTGAGGGTGATGCTGT
a

Forward primer encoding a truncated protein for epitope 1B5.

b

Forward primer encoding a recombinant protein (aa 371 to 570) for epitope 3E8, with aa P373 and H374 mutated to R.

c

Reverse primer for 10 mutation and truncation proteins from avian HEV ORF2.

d

Letters in italics indicate BamHI or XhoI sequences.