FIG 3.

Coimmunoprecipitation of RFXAP with the LANA truncation spanning aa 1 to 150. (A) HEK293T cells were transiently transfected with the Flag-tagged pA3F vector (control), LANA-Flag, LANA-N–Flag, or LANA-C–Flag along with RFXAP-Myc. Cell lysates were subjected to immunoprecipitation with anti-Flag antibody, and the immunoprecipitated proteins were detected with anti-Flag and anti-Myc antibodies after resolving proteins on SDS-PAGE gels and Western blotting. HC represents the heavy chain of the antibody. (B) HEK293T cells were cotransfected with RFXAP-Flag and either NLS-GFP-Myc (vector control), the LANA truncation spanning aa 1 to 340 (LANA 1-340)-GFP-Myc, LANA 1-250–GFP–Myc, or LANA 1-150–GFP–Myc. Cell lysates were subjected to IP using rabbit anti-Myc antibody, and the immunoprecipitated proteins were resolved on SDS-PAGE gels, followed by detection with mouse anti-Myc and anti-Flag antibodies, in order to avoid detection of the heavy chain that comigrates with the LANA region spanning aa 1 to 150.