FIG 4.

Receptor-dependent differences in recA75/17^rfp and recA75/17^rfp H-Y539A infection profiles. (A and B) Vero, Vero-cN4, and Vero-cSLAM cells were infected with recA75/17^rfp or recA75/17^rfp H-Y539A at an MOI of 0.01. Representative fields of view were captured at 1, 3, and 5 days postinfection with a confocal microscope (magnification, ×100; FluoView FV1000; Olympus). ND, not determined (because the cell culture was fully lysed). (C) Growth curves of wt and cN4^blind viruses in different cell lines. recA75/17^rfp and recA75/17^rfp H-Y539A (renamed recA75/17^rfp H-cN4^blind) growth curves were obtained by infection of Vero, Vero-cSLAM, and Vero-cN4 cells at an MOI of 0.01. Cell-associated viruses were harvested at 0, 12, 24, 36, 48, and 60 h postinfection (hpi), and titration experiments were performed in Vero-cSLAM cells. Means ± standard deviations of data from three independent experiments performed in triplicates are shown. To determine the statistical significance of differences between the wt and the mutant viruses' data sets, unpaired two-tailed t tests were performed (*, P < 0.05; **P < 0.01; NS, not significant).