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The main processing steps are illustrated on a representative image. (A) Original grayscale image, (B) high threshold image, (C) low threshold image, (D) processed low threshold image, (E) intersected image, (F) circular objects from the intersected image, (G) dark ring image, and (H) final processed image. Boxed insert shows irregularly shaped objects that are falsely labeled as lipid droplets (LDs) in (E).
