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(A) Fluorescent signal measured for different volumes of Presto Blue solution for 100,000 cells in a 2D static setup (n=3). (B) Fluorescent signal measured for different cell numbers in 2 mL Presto Blue solution in a 2D static setup (n=3). (C) Presto Blue signal in function of incubation time for different time points during 3D static culture and corresponding cell number based on DNA measurements (n=3).
