FIG. 4.

Quantifying the number of lumens formed as a function of aggregate size. (A) 40× phase-contrast microscopy images of MDCK cell aggregates transferred to Matrigel through Sacrificial Micromolding 1 h after transfer. Scale bars are 100 μm. (B) 40× confocal microscopy images of MDCK microtissues fixed and stained with DAPI (blue) and Phalloidin (green) after 4 days of culture. (C) Quantification of cluster diameter (n=30) as a function of the diameter of the PDMS posts used to imprint the sacrificial gelatin microwells (all PDMS posts had a height of 80 μm). (D) Quantification of the number of lumens formed as a function of cluster diameter (n=11). Color images available online at www.liebertpub.com/tec