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Fabrication of tube-in-stent valve. (A) Mold components and tubular mesh with silicone connectors; (B) tubular construct placed in a polymethylmethacrylate (PMMA) holder for static cultivation; (C) tube-in-stent seen from the vascular, (D) ventricular and (E) longitudinal side after insertion into a silicone conduit featuring the sinuses of Valsalva.
