Skip to main content
. Author manuscript; available in PMC: 2015 May 25.
Published in final edited form as: Gastroenterology. 2011 Jul 30;141(5):1749–61.e1. doi: 10.1053/j.gastro.2011.07.038

Figure 4. Neurotensin regulates miR-21 and miR-155 signaling pathways in colon cancer cells.

Figure 4

(A) Luciferase activity of the PTEN and SOCS1 3′UTRs after NT treatment (100nM) for 24h in HCT116 and DLD1 cells (untreated or as-miR-NC treated or as-miR-21-treated or as-miR-155-treated). (B) PTEN and SOCS1 mRNA levels, assessed by real-time PCR, in NT-treated (100nM, 6h) HCT116 and DLD1 cells (untreated or as-miR-NC treated or as-miR-21-treated or as-miR-155-treated). (C) PTEN, SOCS1 and β-actin protein levels, assessed by western blot, in NT-treated (100nM, 6h) HCT116 and DLD1 cells. (D) AKT phosphorylation (S473) levels, assessed by ELISA, in HCT116 and DLD1 cells transfected with as-miR-NC (100nM) or as-miR-21 (100nM) and/or as-miR-155 (100nM) for 24h and treated with NT (100nM) for 24h.