Table 2.

Calculated change in the free energy of stability for each designed synthatase compared to the reference pBpa synthetase (ΔG shown in kcal/mol). The five synthetase enzymes designed to bind ligands shown in Figure 2 correspond to the five rows of this table. Only the design for 5 (ManOTyr) is predicted to have a thermally stable native state and the others are predicted to be unstable.

Synthatase Name	ΔΔG	N Mutations
8 Ala	8.4	8
design for 4	9.8	6
design for 5	−0.2	3
design for 7	4.9	10
design for 2	13.8	9