Table 1.

Maximal blood pressure effects at 3 mg kg⁻¹ body weight and ED₅₀ values of wild-type and knockout mice in response to P2Y_2/4 receptor activation

	Mean arterial blood pressure at baseline (mmHg)	Heart rate at baseline (min−1)	Maximal decrease in %, early phase	ED50 decrease early phase (mg kg−1)	Maximal increase in %, late phase	ED50 increase late phase (mg kg−1)
WT	93 ± 3	489 ± 11	−38 ± 1	0.4 ± 0.1	+44 ± 11	0.5 ± 0.2
P2Y2−/−	111 ± 3*	489 ± 19	+18 ± 1*	0.4 ± 0.1	–	–
eNOS−/−	131 ± 2*	479 ± 17	−42 ± 2	0.3 ± 0.1	+22 ± 3*	0.2 ± 0.1
KCa3.1−/−	91 ± 6	480 ± 28	−13 ± 5*	0.6 ± 0.2	+17 ± 7*	0.3 ± 0.1
KCa1.1−/−	108 ± 2*	548 ± 23	−44 ± 3	0.4 ± 0.1	+38 ± 7	0.5 ± 0.2
Cx37−/−	92 ± 4	501 ± 31	−27 ± 1*	0.6 ± 0.1	+13 ± 3*	0.3 ± 0.1
Cx40−/−	111 ± 5*	465 ± 33	−41 ± 2	0.3 ± 0.1	+33 ± 3	0.4 ± 0.1

Values are mean ± SEM. WT, wild-type mice; P2Y₂^−/−, P2Y2 receptor knockout mice, eNOS^−/−, endothelial NO synthase knockout mice; Cx37^−/−, connexin 37 knockout mice; Cx40^−/−, connexin 40 knockout mice; KCa1.1^−/−, big-conductance potassium channel knockout mice; KCa3.1^−/−, intermediate-conductance potassium channel knockout mice. Additional analysis of previously published data (Rieg et al. 2011) from eNOS^−/− and P2Y2^−/− are included. In contrast, P2Y₂^−/− mice show an acute blood pressure increase in the early phase and lack a biphasic response therefore not data are shown for the late phase. n = 4–8;

^*P < 0.05 vs. WT (one-way ANOVA followed by Bonferroni test for blood pressure, heart rate and ED₅₀ values, two-way ANOVA with repeated measures followed by Dunnett’s test for maximal responses).